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Statistical Analysis In line with EMA, FDA and PMDA guidelines, a crossover design was chosen to leverage the lower within-participant variability in PK measures and thereby limit the number of participants required for the study [28,29,30]

Passaging was performed every three days with Accutase (Sigma-Aldrich)

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This biological process is especially evident in geese which may be anorexic for many weeks, and in king penguins where fasting can extend up to six months [51]