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A third sample (~10 mg) was immediately placed in ice-cold mitochondrial isolation medium upon removal (100 mM sucrose, 100 mM KCl, 50 mM Tris-HCl, 1 mM KH 2 PO 4 , 100 M EGTA, 0.1% BSA

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We explained that without the ability to report an ACO participant's CHOW and effectuate a change in the ACO's participant list during the performance year, the ACO may be unable to provide coordinated care to an ACO participant's patient population, which may cause the ACO's beneficiary count to fall below 5,000