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Signals were developed with SuperSignal West Femto Maximum Sensitivity Substrate (34096, Thermo Fisher Scientific), detected with a Fusion FX System (Vilber, Eberhardzell, Germany), and quantified with ImageJ

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Smokers skin may be more likely to experience palmoplantar pustulosis, an inflammatory condition involving painful blisters on the hands and feet similar to psoriasis that may come back over time

95 Only one study was stratified by histologic cell-type and found that the improvements in the AUC were highest in adenocarcinoma (AUC = 0.81) as opposed to squamous cell carcinoma (AUC = 0.79)

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