After the cells reached 80% confluence, sample preparation for western blotting was performed using lysis buffer [50 mmol/l HEPES, 50 mmol/l NaCl, 1% (v/v) Triton X-100, 10% (v/v) glycerol, 1.5 mmol/l MgCl2, 1 mmol/l EDTA, 10 mmol/l sodium pyrophosphate, 1 mmol/l Na3VO4, 100 mmol/l NaF, 30 mmol/l 2-(p-nitrophenyl) phosphate, 1 mmol/l phenylmethylsulfonyl fluoride, 10 mg/ml leupeptin and 10 mg/ml aprotinin (pH 7.4)] as previously described (39)
So they bought whatever Amazon recommended, got no results, and assumed glutathione doesnt work for them. It wasnt them
Quercetin also increased the percentage of Treg cells and the levels of IL-10 and Foxp3 and decreased the levels of TNF-, TGF-, IL-6, and IL-17 and the percentage of Th17 cells
T., et al
(J Med Chem 2021) characterized the C-20 fatty diacid spacer that extends the half-life